NOTCH4 antibody (AA 1778-2003)

Catalog No. ABIN291848

The Goat Polyclonal anti-NOTCH4 antibody has been validated for WB, ELISA and ICC. It is suitable to detect NOTCH4 in samples from Human.

Quick Overview for NOTCH4 antibody (AA 1778-2003) (ABIN291848)

Target: NOTCH4 (Notch 4 (NOTCH4))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This NOTCH4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC)

Product Details anti-NOTCH4 Antibody

Binding Specificity: AA 1778-2003

Specificity: Recognizes human Notch4.

Purification: Affinity purified

Immunogen: E. coli-derived rhNotch-4 (aa1778-2003).

Isotype: IgG

Application Details

Application Notes: Approved: ELISA (0.5 - 1 μg/mL), ICC (10 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: Western Blot: 0.1-0.2 μg/mL with the appropriate secondary reagents to detect human Notch4 intracellular domain. The detection limit for rhNotch4 intracellular domain is approximately 5 ng/lane under non-reducing and reducing conditions. Immunocytochemistry: 10 μg/mL to detect human Notch4 in serum starved HUVEC cells stimulated with Jagged 1. Cells were fixed with PBS containing 4 % paraformaldehyde for 20 minutes at RT and blocked with PBS containing 10 % normal donkey serum, 0.1 % Triton X-100, and 1 % BSA for 45 minutes at RT. After blocking, cells were incubated with diluted primary antibody overnight at 4?C followed by Rhodamine Red-coupled anti-goat IgG at RT in the dark for one hour. Between each step, cells were washed with PBS containing 0.1 % BSA. Direct ELISA: 0.5-1.0 μg/mL with the appropriate secondary reagents to detect human Notch4 intracellular domain. The detection limit for rhNotch4 intracellular domain is approximately 0.3 ng/well.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: deionized water

Concentration: Lot specific

Buffer: Lyophilized.

Target Details for NOTCH4

Target: NOTCH4 (Notch 4 (NOTCH4))

Alternative Name: NOTCH4

Background: Name/Gene ID: NOTCH4

Synonyms: NOTCH4, HNotch4, Notch (Drosophila) homolog 4, Notch homolog 4 (Drosophila), Notch homolog 4, INT3, Notch 4

Gene ID: 4855

UniProt: Q99466

Pathways: Notch Signaling